Determination of the role of systemic inflamation in osteoarthritis patients with obesity

Abstract

The aim – optimization diagnosis and treatment of osteoarthritis (OA) in combination with abdominal obesity by examining the level of inflammatory markers in patients with comorbidity against the background of complex treatment with the addition of a basic drug therapy (BT) with quercetin and L-arginine hydrochloride.

Material and methods. The study involved 76 patients with OA combined with obesity who were hospitalized in the Rheumatology department of the Ivano-Frankivsk Regional Hospital from 2011 to 2013. To study the effectiveness of proposed treatments, patients were divided into groups: I group – were surveyed in traditional pathogenetic therapy; Group II – patients to traditional pathogenetic therapy added antioxidant quercetin preparation (Corvitin); III Group took traditional pathogenetic therapy with the addition of the aminoacid L-arginine hydrochloride
(tivortin); IV group received traditional pathogenetic therapy in combination with quercetin and L-arginine hydrochloride. To assess the cytokine profile were tested for TNF-α standardized ELISA using a set of reagents ELISA-TNF-α (DIACLONE, France).

Results. The results confirmed that one of the most significant non-genetic risk factors of primary OA is excess body weight, which causes excessive stress on the joints. In the study of indicators of systemic inflammation we found increase in proinflammatory cytokines content in all groups of patients, but rates were highest in patients with comorbidity. It should be noted that the comorbidity concentration of  roinflammatory cytokines was significantly higher than in OA patients, which was confirmed by significant increase of TNF-α content 3.5 times in patients with OA and abdominal obesity. Given that endogenous inflammatory marker which is TNF-α, an important link in the pathogenesis of OA with abdominal obesity, we studied the effect of quercetin and L-arginine hydrochloride in these figures. Although none of the subgroups studied TNF-α reached normal levels, but combined treatment with the addition to traditional therapy quercetin and L-arginine hydrochloride in patients with OA has a pronounced positive effect on cytokine status (p<0.001).

Conclusions. In the study of anti-inflammatory properties of complex treatment (traditional pathogenetic therapy, quercetin, L-arginine hydrochloride), we found a positive effect of proposed therapy on the level of TNF-α.